Basic Cell Culture Protocols
by Cheryl D Helgason;Cindy Miller (9781588295453)

Basic Cell Culture Protocols
 
Cheryl D Helgason;Cindy Miller
Release Date: 10 December 0150
Format: Mixed media product
Pages: 384
Category: Cytology
Publisher: Humana Press
ISBN: 9781588295453
ISBN-10: 1588295451



In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).

AUD $204.95

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