Endometrial Receptors Mediate Spheroids-Endometrium Attachment
by Dr Haggar Harduf (9783639175547)

Endometrial Receptors Mediate Spheroids-Endometrium Attachment
 

Dr Haggar Harduf


Release Date: 01 July 2009
Format: Paperback
Pages: 60
Category: Microbiology
Publisher: VDM Verlag
ISBN: 9783639175547
ISBN-10: 3639175549



Implantation in humans involves cross talk between an active blastocyst and receptive endometrium. The role of the endometrial receptors in this complex embryo-maternal interaction is still unclear. We tested gene and protein expression of endometrial receptors (Plexin-B1, c-Met and Progesterone receptor A) and the effect of these receptors in endometrial receptivity. Methods: Two endometrial cell lines were used: HEi1A and RL95-2 considered as being of low and high receptivity, respectively. Western blot and RT-PCR analysis were utilized to study the receptor expression profile. The role of endometrial receptors in endometrial receptivity was studied by attachment and invasion assays of JAR spheroids on endometrial cells. Different manipulations of inhibition and stimulation of the endometrial receptors were used. Conclusion: Differential endometrial receptor profiles are expressed during the receptivity period. The attachment and invasion processes are separately regulated. We suggest a biologically functional role for PB1, PRA and the complex PB1-c- MET in endometrial receptivity and in the attachment process.

AUD $110.95

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