Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols with CDROM
by Vladimir V Didenko (9781588293800)

Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols with CDROM
 
Vladimir V Didenko
Release Date: 01 January 2006
Format: Hardback
Pages: 372
Category: Biochemistry
Publisher: Humana Press
ISBN: 9781588293800
ISBN-10: 1588293807



The first comprehensive treatment of energy transfer (ET) nucleic acid probes. Hands-on experts thoroughly describe all the major probes, both fluorescence resonance energy transfer (FRET)-based and non-FRET-based, and provide a complete set of techniques to monitor DNA and RNA reactions, including hybridization, amplification, cleavage, folding, and associations with proteins, other molecules, and metal ions. Optimal design strategies for customized ET probes are presented, as well as techniques for distance determination in protein-DNA complexes and the detection of topological DNA alterations, mutations, DNA breaks and single nucleotide polymorphisms. Merging work on nanotechnology, and fluorescent probes, authors describe in detail the design and application of ET-using molecular devices, such as biosensors, molecular machines, and logic gates for molecular scale computation.

AUD $245.95

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