Lq Interaction with E. Coli RNA Polymerase for Antitermination Regions of E. Coli RNA Polymerase Required for Lq-Mediated Antitermination in Binding a
by Heeyoun Bunch (9783639165814)

Lq Interaction with E. Coli RNA Polymerase for Antitermination Regions of E. Coli RNA Polymerase Required for Lq-Mediated Antitermination in Binding a
 

Heeyoun Bunch


Release Date: 01 June 2009
Format: Paperback
Pages: 176
Category: Microbiology
Publisher: VDM Verlag
ISBN: 9783639165814
ISBN-10: 3639165810



Phage Q protein is a transcriptional antiterminator that is required for late gene expression from the pR' promoter when prophage decides to follow the lytic pathway. Q modifies Escherichia coli RNA polymerase (RNAP) at the early elongation stage. Once engaged, Q maintains itself as a subunit of the elongation complex throughout the elongation. Studies show that Q confers RNAP resistance to both intrinsic and -mediated terminators and less pausing. However, little is known about the interaction between Q and RNAP. In this study, the largest subunits ( and ') of RNAP were examined to map the Q binding site. A stronger binding was observed with than ', and the binding site was narrowed to 501-832. This region was further defined as small as 82 amino acids (600-681) that interacted with Q. In addition, 12 mutant RNAPs were isolated for a reduced Q antitermination within the potential Q binding regions, specifically near the main channel. These results propose that Q modifies the active center of RNAP for antitermination.

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