Probing Protein Adsorption on Model Surfaces
by Francis Nsiah (9783639100594)

Probing Protein Adsorption on Model Surfaces
 

Francis Nsiah


Release Date: 10 December 1010
Format: Paperback
Pages: 124
Category: Chemistry
Publisher: VDM Verlag
ISBN: 9783639100594
ISBN-10: 363910059X



The ability to control nonspecific protein adsorption at interfaces is a key issue in the design of biosensors, assays, chromatographic separations and implant materials. The research presented in this manuscript focuses on the effect of surface composition on the properties of adsorbed protein layers and the probing of protein-protein interactions both on adsorbed surfaces and in solution. Monolayers of alkanethiols on gold formed from solution self-assembly and microfluidic patterning using poly(dimethyl) siloxane (PDMS) served as platform for protein adsorption. We are employed surface plasma resonance (SPR) imaging and infrared reflection absorption spectroscopy (IRRAS) to probe the conformation and orientation of adsorbed proteins, as well as, the characterization of protein layers.

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